This study investigates the occurrence of all priority substances (n = 41) listed in the Water Framework Directive and additional substances (n = 47) in raw sewage, as well as the removal performance of lamella clarification and biofiltration techniques. Once the efficiency of both types of techniques has been assessed for typical wastewater parameters, the differences in each technique's ability to remove pollutants becomes obvious; nevertheless, pollutant removal in quantitative terms still depends on the physico-chemical properties of the compounds used and operating conditions within the selected facility. For lamella clarification, the removal of organic chemicals was found to be primarily correlated with their sorption potential and, hence, strongly dependent upon log Kow of the compound under study. Compounds with a strong hydrophobic character (log Kow > 4.5) are removed to a significant extent (approx. 85%), while hydrophilic compounds (log Kow < 3.5) are poorly removed (< 20%). For biofiltration, the removal of chemicals appears to be compound-dependent, although this outcome involves several mechanisms, namely: i) physical filtration of total suspended solids, ii) volatilisation, iii) adsorption on biomass, and iv) biotransformation of substances. Even if the complex processes within a biofilter system do not yield an accurate prediction of pollutant removal, two groups of chemicals can still be clearly identified: i) hydrophobic or volatile compounds, for which moderate to high removal rates are observed (from 50% to over 80%); and ii) hydrophilic, non-volatile and refractory compounds for which a low removal rate would be expected (< 20%).